MCM10 Knockout cell line(KYSE-150)

EN CN
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location： Home > Products > KO Cell Lines > MCM10 Knockout cell line(KYSE-150)

     gRNA Plasmid Bank

     Cell Lines

KO Cell Lines

Wild-type Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cas9 Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Luciferase Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

EGFP Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Stem Cell Lines

     Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

     Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

     Lentivirus

gene KO/PM cell lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

MCM10 Knockout cell line(KYSE-150) Order

MCM10 Knockout cell line(KYSE-150)
Catalog#
YKO-HJ090
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MCM10 Knockout cell line(KYSE-150)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MCM10 Knockout cell line (KYSE-150)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
MCM10
Gene ID
55388
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_E0WU(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript MCM10-202)
Validation: PCR+Sanger Sequencing
MCM10 Knockout cell line(KYSE-150) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:KYSE-150		 STR Info (Cell bank)
Cell Line:KYSE-150
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 811811
D7S820 10111011
D16S539 911911
VWA 16171617
TH01 7979
AMEL XXXX
TPOX 8888
CSF1PO 12131213
D12S391 1922
FGA 2124
D2S1338 2525
D21S11 3031
D18S51 1414
D8S1179 1015
D3S1358 1516
D6S1043 1820
PENTAE 1218
D19S433 1515
PENTAD 1010
D1S1656 1517.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MCM10 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the KYSE-150 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MCM10 gene knockout MCM10 Knockout cell line (KYSE-150) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MCM10 Knockout cell line(KYSE-150)
MCM10 Knockout cell line(KYSE-150)
Catalog#
YKO-HJ090
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • MCM10 Knockout cell line(KYSE-30)

    MCM10 Knockout cell line(KYSE-30)
      inquiry   More details

    Organism: Human
    Gene Name: MCM10
    Gene ID: 55388
    Size: 1*10^6
    Catalog#: YKO-HJ089
    YKO-HJ089(KYSE-30)

Related Popular Products

Wild-type Cell Lines

  • KYSE-150 cell line

    Human Esophageal Squamous Carcinoma Cell Line(KYSE-150)

    Price (USD): -
    Catalog#: YC-B002
    Size: 1*10^6
    Instruction: Human Esophageal Squamous Carcinoma Cell Line(KYSE-150)
      inquiry   More details

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • MCM10 Knockout cell line(KYSE-30)

    MCM10 Knockout cell line(KYSE-30)

    Organism: Human
    Gene Name: MCM10
    Gene ID: 55388
    Size: 1*10^6
    Catalog#: YKO-HJ089
      inquiry   More details

WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
Contact us